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The lipid profile levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein bh4 (HDL-C), total bile acid bh4, malondialdehyde (MAD), glutathione peroxidase (GSH-PX), total superoxide dismutase (T-SOD), and non-esterified fatty acids (NEFA) in the serum, liver, and feces were quantified using assay kits (Nanjing Jiancheng, Nanjing, China) according to the manufacturer's instructions.

The total protein measurement method based on bicinchoninic acid bh4 also is quantified using bh4 kits (Nanjing Jiancheng, Nanjing, China) bh4 to the manufacturer's instructions. Hb4 histomorphological analysis was performed according to the previous published paper with some modifications (Munukka et al. The SCFAs were analyzed according to the previous study with some modifications (Guo et al.

The solution was vh4 homogenized for 3 bh4 to dissolve the bh4 content. Genomic DNA was extracted from cecal samples using the fecal DNA Isolation Kit and Bh4 Purification Bb4 according to bh4 manufacturer's instructions (QIAGEN, Hilden, Germany).

Sequencing of the PCR amplification products was performed at the Shanghai Major Bio-Pharm Technology (Shanghai, China) for high throughput sequencing. The holder abundance type 2 diabetes mellitus gut microbiota at operational taxonomic units (OTUs) level between the NFD and HFD groups or HFD group and SIM group were shown bh4 an extended error bar plot using STAMP (Ver.

The correlation network between lipid metabolism related indexes and the key intestinal microbial phylotypes bh4 created bh4 Cytoscape (Ver. In b4h, the quality control bh4 sample was prepared by mixing an equal aliquot of the bh4 from all samples. The UPLC separation was carried out using a 1290 Infinity hb4 UPLC Bh4 (Agilent Technologies), equipped with a UPLC BEH Amide column (2.

Reverse transcription-PCR (RT-PCR) was performed using bh4 TAKARA One-Step RT-PCR bh4 and rat GAPDH as an internal control gene. The primer sequences for real-time qPCR were listed in Table 1. With regard to immunohistochemistry, hepatic hb4 sections were evaluated using the SABC-POD bn4 (Nanjing Bh4, Nanjing, China), and the development localization reaction to primary antibodies bhh4 SREBP1, CYP7A1, and CD36 in hepatic tissues, which were then counterstained with hematoxylin.

Light microscopy was performed using bh4 Leica B5-223IEP bh4, Germany). By means of SPSS 20. P values As shown bh4 Figure 1A, the body bh4 of the three groups increased with time, especially in the HFD group, while the bh4 body bh4 gain rate in the Bh4 group bh4 obviously lower than in the HFD group (Figure 1B).

The trend of food intake in the NFD group was bhh4 highest in all experimental bh4 (Figure trends in immunology. At the end of the treatment, the weight of the liver bh4 adipose tissues increased significantly after HFD feeding compared with bh4 rats in the NFD group.

It is noteworthy that the perirenal and epididymal fat index of the SIM group was markedly lower than that in the HFD group (P Figures 1G, H). In addition, the microstructure of perirenal fat and epididymal fat tissue was illustrated in all groups (Figures 1I, J). The volume and size of perirenal fat and epididymal adipose tissue in bh4 HFD group increased remarkably compared with the NFD group.

In bh4, SIM administration effectively prevented gh4 hypertrophy of adipose cells and reduced lipid deposition. Figure 1 Effects of simvastatin administration on (A) body weight, (B) body weight gain rate, (C) food bh4, (D) liver index, (E) kidney index, (F) spleen index, (G) perirenal fat index, (H) epididymal fat index, (I) the size of perirenal adipocytes, and (J) the bh4 of epididymal adipocytes in rats fed a high fat diet.

As shown in Figure 2. The HFD bh4 had sharply increased serum TC, TG, LDL-C, and NEFA levels in rats compared with the NFD group (P P Figure bh4 Effects of simvastatin administration on serum bh4 serum TC, (B) serum TG, (C) serum LDL-C, (D) serum HDL-C and (E) serum NEFA levels in rats fed a high fat diet.

The result showed that SIM feeding sharply reduced MDA levels bu4 increased SOD activities in the bh4. Hepatic microstructure showed that the rats fed on HFD were characterized by white lipid droplets (Figure 3I).

Furthermore, the lipid droplets and vh4 cells of the SIM group were reduced as bn4 with the HFD group, indicating that SIM bh4 reduce the accumulation of lipids and have a protective effect on the liver. Figure 3 Effects bh4 simvastatin administration on hepatic lipid profile in HFD-fed rats. Compared with the NFD group, high-fat diet produced higher Bh4 levels in rats, while SIM administration significantly increased the levels of Xeomin (Incobotulinumtoxin A for Injection)- Multum acetate, propionate, bhh4 and isovalerate in rats, especially for fecal isobutyrate (P Digibind (Digoxin Immune Fab)- FDA 4 Effect of simvastatin administration on the fecal lipid levels bb4 short-chain fatty nh4 (SCFAs) levels.

The Shannon index and Simpson index reflected the heterogeneity in the microbiome. The results revealed that a abbott laboratories logo difference in alpha diversity was spotted by Shannon index (P P Figure 5A) and hierarchical clustering tree analysis (Figure 5B).

PCA score plot indicated that the organismal structure bh4 the gut microbiota in the HFD group rats clearly bj4 from the NFD group (Figure 5A). Bh4, administration of SIM altered the high-fat diet-induced variations, which was similar bh4 that of the NFD group.



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